Development and validation of an RP-HPLC method for determination of Metformin and Evogliptin in bulk and pharmaceutical dosage forms

Introduction and Aim: In this study, a new sensitive rapid HPLC method was developed to determine Metformin hydrochloride Evogliptin tartrate in pharmaceutical dosage forms. Materials Methods: Chromatographic separation of achieved on Waters Alliance-e2695 by using XTerra RP-18 150X4.6 mm, 3.5µ column, the mobile phase containing Acetonitrile: KH2PO4: Methanol ratio 50:40:10% v/v. Results: The flow rate 1.0 ml/min; detection carried out absorption at 228 nm photodiode array detector ambient temperature. Conclusion: This simple, economical, suitable, precise, accurate robust for quantitative analysis study its stability.